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PhD-student position in Molecula Plant Microb InteractionsPosted by: Shanghai Jiaotong University
Posted date: Nov-27-2019
Shanghai Jiao Tong University (SJTU), as one of the higher education institutions which enjoy a long history and a world-renowned reputation in China, is a key university directly under the administration of the Ministry of Education (MOE) of the People's Republic of China and co-constructed by MOE and Shanghai Municipal Government. Through some 120 years' unremitting efforts, SJTU has become a comprehensive, research-oriented, and internationalized top university in China.
For international PhD student, we have four kinds of scholarship. Details can be found here: http://www.agri.sjtu.edu.cn/En/Data/List/SAB
To apply the position, please follow this link:
Introduction to our lab:
RNA editing is a posttranscriptional event that alters genomic RNA sequences via insertion, deletion, deamination or substitution. Among these methods, adenosine (A)-to-inosine (I) RNA editing, which is catalyzed by the adenosine deaminase acting on RNA (ADAR) family of enzymes, is the most prevalent type of editing in metazoans. This form of editing has been confirmed to play an important role in various biological processes ranging from nonsense-mediated mRNA decay and alternative splicing to gene expression and translation. Because I is recognized as guanosine (G) by the polymerase enzyme and translational machinery, A-to-I editing in coding regions of mRNA may lead to codon changes, resulting in diversification of protein function. The importance of A-to-I editing in eukaryotes has been confirmed, and this editing can occur in both noncoding and coding regions. Nevertheless, in prokaryotes, A-to-I RNA editing of mRNA has rarely been reported.
In our group, we will use model rice bacterial pathogens to check the relationship between innate immune recognition and A-to-I mRNA editing at the level of pathogen-associated molecular pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) by overexpressing or knock-out genes, pull-down assay, Co-IP, GUS reporter assay, electrophoretic mobility shift assay (EMSA) and microscale thermophoresis (MST).
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2. Zhu B, Muhammad Ibrahim, et al. 2016. Multi-omics analysis of niche specificity provides new insights into ecological adaptation in bacteria. ISMEJ.10:2072-2075.
3. Ji Z.Y., Ji C. H., Liu B., Zou L.F., Chen G.Y., and Yang B. Interfering TALEs of Xanthomonas oryzae neutralize the R-gene-mediated plant disease resistance. Nat Commun, 2016, OI: 10.1038/ncomms13435.
4. Li Z., Zou L., Ye G., Xiong L., Ji Z.Y.,Zakria M., Hong N., Wang G.P., and Chen G.Y. A potential disease susceptible gene CsLOB of citrus is targeted by a major virulence effector PthA of Xanthomonas citri subsp. citri. Mol Plant, 2014, 7(5):912-915.